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For hematocrit and reticulocyte counts, recommendations are to repeat these tests daily from the 5th to 7th days of treatment and then frequently until the hematocrit returns to a normal range (11, 14, 15)

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After amplification, PCR products were electrophoresed on agarose gels and transferred onto nylon membranes using Southern blotting techniques

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Swirl gently or invert slowly until fully dissolved (typically 3060 seconds)