glp-1 dpp 4 Mechanism of action for GIP, analogues and DPP4 inhibitors in DPP-4 Enzyme: The GLP-1 Breakdown

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[DOI] [PMC free article] [PubMed] [Google Scholar] Zheng H, Whitman SA, Wu W, Wondrak GT, Wong PK, Fang D, Zhang DD

GLS1, glutaminase 1

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For antibody labeling, after treatments at 37 C, cells were placed on ice to arrest further endocytosis before fixation, and the surface GLP-1R was detected by ELISA with monoclonal anti-human GLP-1R antibody 68 (Mab 3F52, Developmental Studies Hybridoma Bank (DSHB), 1/100) plus horseradish peroxidase (HRP)-conjugated rabbit anti-mouse secondary (ab97046, Abcam, 1/5,000)

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