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10.1007/s12013-011-9199-z 19 LiS.ZhongS.ZengK.LuoY.ZhangF.SunX.et al (2010)

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Proteins were then separated via SDS-PAGE and transferred to PVDF membranes, which were blocked with 5% skim milk for 1 h at 25 C before incubation with primary antibodies for 12 h at 4 C