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doi:10.4049/jimmunol.159.2.574 17 SrinivasanVMaestroniGJCardinaliDPEsquifinoAIPerumalSRMillerSC

Since the colorimetric reaction starts immediately after the addition of Substrate working solution to a well, use a multichannel pipette to avoid the reaction time lag of each well

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The more useful question is not only how much, but how much, how often, and why that schedule. A practical comparison helps here

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A consistent 5x/week loading schedule outperforms sporadic 7x/week dosing with missed days scattered throughout