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doi:10.1007/s00125-024-06321-2 43

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Then, 100 L of this combination was evenly spread onto microscope slides, which were immersed in a lysis solution made of NaCl (2.5 M), EDTA (100 mM), TRIS buffer (10 mM), 1% lauryl sarcosinate, 1% Triton X-100, and 10% DMSO for 15 min