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Buthionine sulfoximine (BSO) is a glutathione depleting agent that inhibits the enzyme gamma-glutamylcysteine synthetase which is required in the early steps of glutathione synthesis

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J Gastroenterol Hepatol 7:711 Barbaro G, Di Lorenzo G, Soldini M, Parrotto S, Bellomo G, Belloni G et al (1996) Hepatic glutathione deficiency in chronic hepatitis C: quantitative evaluation in patients who are HIV positive and HIV negative and correlations with plasmatic and lymphocytic concentrations and with the activity of the liver disease

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Glucagon biology can be metabolically useful in the right context, but it is not as straightforward as GLP-1 appetite suppression

Finally, stroke especially affects the elderly, and additional studies in older mice should be performed