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After 2.5 h, PDX tumors were harvested, and weigh 20-30 mg for metabolite extraction by 80% methanol with 2 M myristic acid

(2019) 'Mild depletion of vitamin B2, B6 and B12 superimposed on mild folate depletion: Effects on DNA methylation, uracil incorporation and gene expression in the mouse colon', *Scientific Reports*, 9, p

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This variability emphasizes the need for personalized medicine, where Se therapy is tailored according to an individuals genetic makeup, metabolic profile, and thyroid health status [103]

Consistently, GSH depletion using BSO impairs SLC31A1-mediated copper uptake, underscoring its requirement for efficient intracellular copper delivery 198

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