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10.1093/humrep/deg450

pp.449-456

Finally, the beads were washed 3 times with 1 ml of modified binding buffer (125 mM Tris-HCl (pH = 8.0), 250 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1% Tween 20)

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