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Therefore, our results describing the new analgesic mechanism underlying the action of acetaminophen on the spinal dorsal horn, are reasonable compared to previous reports ( Acetaminophen does not possess any anti-inflammatory activity, because it is a very weak inhibitor of COX and does not inhibit neutrophil activation ( The purpose of this review was to summarize the previous and new issues related to the analgesic mechanisms of acetaminophen and discuss our understanding that acetaminophen metabolite AM404 also acts on the spinal dorsal horn and induces analgesia in inflammatory pain conditions

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Then, 1% TBA was added, and the samples were boiled (95 C, 60 min)

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