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The kidney is suggested to have the highest levels of GGT1 (ref
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When incorporated into proteins, histidines side chain can act as a proton acceptor and donor, conveying important properties when combined into enzymes such as chymotrypsin and those involved in the metabolism of carbohydrates, proteins, and nucleic acids
This approach inspired other programmes: N-hexanoic-Tyr-D-Arg : enkephalin-derived, protease-resistant, studied for pain N-methyl-Aib inclusions : N-methyl substitutions increase membrane permeability at the cost of partial loss of receptor affinity Beta-peptides and D-amino acids : substitution of natural chiral residues by their D enantiomers, making the peptide invisible to endogenous proteases while (sometimes) preserving function Stapled peptides : hydrocarbon cyclisation, popularised by Verdine (Harvard), stabilises alpha helices and improves cellular penetration These strategies progressively shift the peptide / small molecule frontier