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CRISPRCas9-mediated gene knockout Knockout of ERN1 (gene name of IRE1) in human cell lines was performed using single guide-RNAs (sgRNAs) based on the pSpCas9(BB)-2A-Puro (PX459) (Addgene Plasmid #48139) vector developed previously 88

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If you are wondering how many carbs are in 2 eggs, the total for two large eggs is approximately 0.96 grams

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Gender differences in Takotsubo syndrome

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