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Accessed May 13, 2025

Dulbeccos modified Eagles medium (DMEM) with 5% fetal bovine serum (FBS) and 1% penicillinstreptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) was used for the culturing of the Raw264.7 cells

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A simpler surrogate is the acetaminophen (paracetamol) absorption test : because acetaminophen is negligibly absorbed by the stomach but rapidly absorbed by the small intestine, the rise in its plasma concentration tracks the rate of gastric emptying a convenient method often embedded in early-phase metabolic studies

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