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Reactive species assays Reactive species were measured using the luminescent probe L-012 (Wako Chemical) and the fluorescent dye fluorescein-boronate (Fl-B) 63

The declining plasma concentration provides a quantitative framework for researchers designing dose-response studies, as physiologically relevant in vitro concentrations should bracket the 200-500 nM range to model the young-to-aged spectrum

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Introducing or modifying amino, carboxyl, and hydroxyl groups enhances solubility, biocompatibility, fluorescence stability, and payload control [33]

The liver is the major organ of the synthesis of endocrine factors including IGF-1 and IGF-2, as well as the binding proteins (IGFBPs) (Voci et al., 1999)