The tissues were rinsed with PBS and incubated with Alexa Fluor488 chicken anti-rabbit (1:1000, Life Technologies) for one hour at 37 C and then rinsed with PBS and incubated with Alexa Fluor488 goat anti-chicken antibody (1:1000, Life Technologies) and Alexa Fluor568 goat anti-mouse antibody (1:1000, Life Technologies) for another hour at 37 C
Caution is also advised in individuals with certain inborn errors of amino acid metabolism or those on extremely high-protein diets, although no specific taurine-related contraindications have been documented to date
Among the module membership was GCL, composed of the catalytic GCLC and the modifier GCLM subunit, shown to be upregulated (Fig
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