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After DNase treatment (AMPD1, Sigma), 1000 ng of RNA was reverse-transcribed using random hexamers and Superscript II reverse transcriptase (Thermo Scientific)

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In the paper ( Regarding bufalin, it stimulates B cell proliferation from leukemic BALB/c mice compared with the leukemic control group at doses 0.1 and 0.2 mg/kg

Two of the most commonly used ingredients are L-arginine and L-citrulline