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Reconstitution & Handling Each 5 mg vial is typically reconstituted with 2 mL of bacteriostatic water, yielding a 2.5 mg/mL working stock, though investigators may adjust volumes based on study design and dosing precision needs

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Methionine synthase catalyzes the transfer of a methyl group to S-adenosyl-homocysteine, generating S-adenosyl-methionine and hence methionine, while methylmalonyl-CoA mutase catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, which then enters the citric acid cycle (Figure 1) [4,5,6]