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Examination of proinflammatory and anti-inflammatory cytokines Proinflammatory cytokines (IL-6, TNF- , and IL-1 ) and inflammatory cytokine (COX2) were determined in serum following the manufacturers protocols of purchased ELISA kits 35

Cells were then washed twice with PBS followed by one wash with ddH 2 O

However, their limited ability to cross the BBB (under 6%) hinders routine formulations from achieving therapeutic concentrations in the brain, significantly restricting their clinical applicability 13

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