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doi:10.1172/JCI28898 17
An unopened vial left at room temperature for 24 hours can be returned to freezer storage with minimal degradation, but repeated temperature excursions compound the damage
Bafilomycin or DMSO was added in the same condition as the wound healing assay 30 min before EV addition, and labeled l-EVs of 2 10 9 particles were added to PANC-1 cells in 200 l of 10% EV-depleted FBS-containing DMEM and incubated for 3 h
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