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Raster Image Correlation Spectroscopy (RICS) image capture and analysis INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto glass bottom MatTek dishes and labelled with SNAP-Surface 647 (S9136S, New England Biolabs) for 15 minutes at 37C in full media, washed and imaged in RMPI-1640 media without phenol red using a 100X oil objective on a Leica Stellaris 8 STED FALCON microscope from the FILM Facility at Imperial College London using confocal settings as previously described (81)

doi: 10.1007/s11095-022-03302-1 50

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Injection Site Reactions with Weekly Weight Loss Injections For patients on injectable treatments, mild redness, swelling, or tenderness at the injection site may occur

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Cardiovascular Benefits: Some GLP-1 medications have shown cardiovascular protective effects, reducing the risk of major adverse cardiac events