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Not priming before injection Skipping the priming step means your first injection might deliver a mixture of air and solution rather than a full dose of peptide

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Thus, it has been determined that increased intracellular calcium and calmodulin activation in the lens can activate connexin hemichannels (essential for mediating communication and the exchange of the molecules between cells, as well as between the intracellular and extracellular environments) in lens fibers, disrupting their proper homeostasis and consequently favoring cataract development [45]