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39.8 wk average gestational age)

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To view a copy of this license, visit About this article Cite this article Sun, Y., Zheng, Y., Wang, C

RT-qPCR For RT-qPCR, more than 100 well-fed synchronised worms were collected into TRIzol Reagent (Invitrogen, 15596018) and column purified by RNeasy Mini (QIAGEN, 74104)

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A drop of a 1% aqueous solution of phosphotungstic acid dye was used to stain the freshly made sample, which was then placed onto the surface of a carbon-coated copper grid for TEM visualization