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This is consistent with the observations that activation of Rac1 during training but not after training impaired long-term memory, and that Rac1 inhibition during LTP induction, not after induction, impaired LTP (Martinez and Tejada-Simon, 2011

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The SmBiT tag was cloned in frame at the C-terminus of the GLP-1R by substitution of the Tango sequence on a FLAG-tagged GLP-1R-Tango expression vector (Kroeze et al., 2015), a gift from Dr Bryan Roth, University of North Carolina (Addgene plasmid # 66295)

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Bromodomain and extraterminal proteins (BET) recognize and bind to acetylated lysines on histone tails, which signals that chromatin is open for transcription