Following centrifugation (20,000 x g, 5 min top remove undigested material), 250 l of each sample was transferred into a new tube containing 250 l of 1 st wash buffer (50 mM TEAB, 150 mM NaCl, 0.2% NP-40) containing 50 l of streptavidin agarose beads that were pre-washed twice by the 1 st wash buffer)
Summary Keywords glutathione metabolism, hippocampal injury, multi-omics, oxidative stress, sepsis-associated encephalopathy Citation Li Y, Wang L, Ma T, Peng T, Wang L, Wang J, Li Y and Wang Y (2026) Glutathione metabolism as a key regulator of oxidative hippocampal injury in sepsis-associated encephalopathy: an integrated proteomics and metabolomics study
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