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Methods Reagents and tools table Methods and protocols Preparation of PDMS substrates with tunable stiffness and ECM protein coating Polydimethylsiloxane (PDMS) substrates were prepared using a SYLGARD 184 Elastomer kit (Dow Silicones, USA) and varying the crosslinker:silicone base agent ratio to 1:60, 1:35, and 1:10, respectively (Fig

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It is an integral membrane protein expressed on cells throughout the body, but is also shed from the membrane and circulates as a soluble protein in the plasma