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Exceeding this time frame can increase the risk of hemorrhagic transformation (Niego et al., 2012)

Fluorescence was measured at 4, 24, 48 and 72 h after treatment at 485 nm excitation and 530 nm emission on a SpectraMax M3 microplate reader (Molecular Devices)

In severe cases, subacute combined degeneration of the spinal cord can occur, affecting both sensory and motor function

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For deficiency treatment, frequency is guided by severity and cause