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Briefly, sitting drop crystallisations were set up in 96-well trays using a Cartesian robot with a reservoir solution of 1.5 M ammonium sulfate and 0.1 M Tris at pH 8.5, each crystallisation drop containing 100 nL each of protein and reservoir solutions

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IGF-1 LR3 and PEG-MGF are listed on the World Anti-Doping Agency (WADA) Prohibited List under category S2 (peptide hormones, growth factors, and related substances), making them prohibited year-round for athletes subject to anti-doping testing

It is therefore difficult to distinguish whether the increase in drug effect due to the above-mentioned particles is a metabolic obstacle or other reasons