For the luciferase assays, 5 L of the supernatant was diluted with 95 L PBS (pH 7.4) in white flat-opaque-bottomed 96-well plates (Thermo Fisher Scientific)
This gives you a Day 1 reference point
This multistep, one-pot process proceeds via the intermediacy of the isothiouronium salt, which is hydrolyzed in a separate step: [24] [25] CH 3 CH 2 Br + SC(NH 2 ) 2 [CH 3 CH 2 SC(NH 2 ) 2 ]Br [CH 3 CH 2 SC(NH 2 ) 2 ]Br + NaOH CH 3 CH 2 SH + OC(NH 2 ) 2 + NaBr The thiourea route works well with primary halides, especially activated ones
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Research documents inflammation markers in TB-500-treated versus control tissues