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COVID-19-associated microvascular damage, such as endothelitis, microthrombosis, capillary damage, damage to pericytes, and fluid buildup in the alveoli, results in hypoxia and reduced SpO2 [97]

Although studies have suggested a role for superoxide dismutase (23), the relationships with redox changes and clinical parameters have generally been weak, supporting involvement of other pathways

An interaction with glutathione/glutaredoxin has been observed in all roGFP-based sensors generated to date 19,35,49,50,51,52,53 and it is unclear why this interaction should not also occur in the context of the NERNST probe

Engineering: This "No DAC" variant features four specific amino acid substitutions (Tetrasubstitution) that protect it from DPP-IV enzymatic cleavage, extending its biological activity to 30-60 minutesideal for mimicking natural physiological pulses

The positive control was treated with 1 mM tert -butyl hydrogenperoxide at 37 C for 1 h

The redox stress hypothesis of aging