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Some antibiotics used in the treatment of these conditions can have a negative impact on liver function

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PCR products were extracted from these gels using a QIAquick Gel Extraction kit (Qiagen)

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Drug treatment varied by age in the present study and in the NASPGHAN recommendations due to expert opinion regarding low tolerability (tachycardia and increased frustration) of amitriptyline in younger children and low efficacy of cyproheptadine in older children [1]