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Cells were washed once in dPBS1X and resuspended in 300 l hybridization mixture (formamide 70%, blocking reagent 1, Tris-HCl, pH7.4, 10 mM, telomeric PNA probe 0.5 mM)

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By combining this experimental structure with a well-controlled crossover design, the study was able to isolate the effects of L-carnitine supplementation