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It could be that a piece of equipment was later found to have malfunctioned or was calibrated incorrectly (Anon, 2018)

Samples were removed from the water bath, 250 NRS was added, and each was placed into a rotisserie hybridization oven at 37C with rotation at high speed for the appropriate incubation time (10, 20, 30 40 or 60 minutes)