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Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator

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UAS-HA-V5-LaccID flies were generated from gBlocks containing a wingless signal peptide upstream of two epitope tags (HA and V5) followed by Drosophila codon-optimized LaccID fused to CD2

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Consistent with this observation, decreased TNF-stimulated IB- phosphorylation was observed in hepatocytes with elevated GSSG levels