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The copper(II) ion (Cu2+) is chelated by the nitrogen atoms of the glycyl-histidyl-lysine tripeptide, specifically the terminal amine, the imidazole nitrogen of histidine, and the deprotonated amide nitrogen between glycine and histidine

Zhou X, Speer RM, Volk L, Hudson LG, Liu KJ

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This MDA-TBA adduct is then measured colorimetrically at 530-540 nm