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These gaps highlight the need for future research to move beyond single-cell type and descriptive mechanisms toward systematic dissection of intercellular crosstalk, renal parenchymal cell contributions, and clinical validation

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Seven of the 19 XChem hits were shown to bind to the protein, but with only a modest average peak intensity reduction of 2037% in the CPMG assay (Supplementary Table 2), suggesting that the fragments likely bound with weak (millimolar) affinities, at the very limit of the CPMG assay sensitivity

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