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Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

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In situ hybridization was performed on the human liver biopsies on a Ventana Discovery Ultra automation system (Ventana Medical Systems) using an RNAscope VS Universal HRP/AP Kit (Advanced Cell Diagnostics) and the specific GLP1R probe, RNAscope 2.5 VS Probe-Hs-GLP1R (Advanced Cell Diagnostics, cat