Inflammation in epileptogenesis after traumatic brain injury
doi:10.1007/s00726-010-0510-x Kranich O, Dringen R, Sandberg M, Hamprecht B (1998) Utilization of cysteine and cysteine precursors for the synthesis of glutathione in astroglial cultures: preference for cystine
Based on prior experience with this kit, to ensure proper mixing of reagents, we placed the 96-well plate on an orbital plate shaker during incubation steps
Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions
2.9 Software and code: All design-based analyses were performed using R version 4.5.1 (2025-06-13 ucrt) and survey library version 4.4.8
New users should begin at lower concentrations (0.5% to 1%) regardless of wrinkle severity to establish tolerance before increasing