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The plant extract was done in an ice-cold buffer containing 50 mM phosphate buffer (pH 6.5) containing 0.1% triton X, 0.2 mM EDTA, 1% (W/V) polyvinylpyrrolidone (PVP), 1% leupeptine, 0.1% DTT, 1 mM proteinase K, 5 mM ascorbate and centrifuged at 10,000 g at 4 C for 20 min

13 15 Figure 20-1 Factors determining bronchial smooth muscle tone

doi: 10.1007/s12011-015-0581-3 57 ChakrabortyA

The goal was to create a peptide that could, in theory, accelerate fat breakdown and inhibit fat storage without impacting blood sugar or causing the acromegaly-like symptoms associated with excessive hGH

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PubMed Central , For your reference: For your reference: For your reference: 15, 25 Mateus, Filipa G., et al