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Instead of overcoming the metabolic adaptation, higher doses frequently exacerbate side effects like nausea, fatigue, and gastrointestinal distress

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The intracellular metabolite extracts and 2 mL of spent culture medium containing extracellular metabolites were freeze-dried (BenchTop K manifold freeze dryer, VirTis) prior chemical derivatisation

meaning if you are functionally deficient in B2, it is highly likely you will also have a B12 functional deficiency