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After deparaffinization and hydration, the sections were treated with heated citrate buffer (pH 6.0), 0.3% H 2 O 2, and blocked with 3% BCA successively, then incubated with primary antibody at 4 C overnight and biotinylated secondary antibody

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Finally, GPX3 as a secreted isoform of GPX (Steinbrenner and Sies, 2009

Change the volume based on the strength