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(3) 72 hours later, the rats are anesthetized and immediately perfused transcardially with 50 mL 0.9% saline followed by 500 mL 0.1 m neutral phosphate-buffered formaldehyde (4%)

Glutathione has two mutually interconvertible forms, reduced glutathione (GSH) and oxidized glutathione (GSSG)

De Sanctis V, Soliman A, Alaaraj N, Ahmed S, Alyafei F, Hamed N

PMCID: PMC5909471

The catalytic tetrad formed by Sec, glutamine, tryptophan and asparagine is essential for GPx activity, since these residues are crucial for enzyme-substrate interaction and stabilization of the GSH-GPx interaction [47, 65, 66]

i think i damaged them a few times over the last few years