Benzyl alcohol increases bacterial cell membrane fluidity and destabilizes membrane structures
With 1 mL, you might get 10-20 draws with smaller volumes each time
Upon activation, NLRP3 engages the scaffold protein NIMA related kinase 7 (NEK7) and recruits the adaptor apoptosis-associated speck-like protein containing a CARD (PYD and CARD domain containing (PYCARD, also known as ASC)) to assemble the inflammasome complex
Ward MG, Kariyawasam VC, Mogan SB, Patel KV, Pantelidou M, Sobczyska-Malefora A, et al
emission 520 nm) was read in the upper and lower chambers, and the permeability coefficient was determined using the following formula: 22 P dextran =( RFU lower / RFU upper )( V )(1/ t )(1/ A ), where RFU is the relative fluorescent units in the upper and lower wells, V is the volume of the bottom well, t is the time that the FITC-dextran was allowed to diffuse, and A is the total surface area of the monolayer (cm 2 )
10.1016/j.ijid.2024.107124 105 YongQ.HuangC.ChenB.AnJ.ZhengY.ZhaoL.et al (2024)