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Membranes were blocked with 5% BSA (##9048-46-8, Sigma-Aldrich, MO, USA) in Tris-buffered saline (##1610716, Bio-Rad, CA, USA) containing 0.05% Tween-20 (##9005-64-5, Sigma-Aldrich, MO, USA) (TBST) for 1 h at room temperature

Varying concentrations of MB or its derivatives and 20 mM glutamate were added to each well and incubated for 12 hours at 37C with 5% CO 2

This formula combines diindolylmethane, calcium D-glucarate , and broccoli seed extract in a convenient vegetarian capsule format