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Briefly, a 24-well culture plate was coated with 200 l (9.1 mg/ml)/well Matrigel prepared from Engelbreth-Holm-Swarm mouse tumors (Corning, MA, USA) and allowed to stand for 30 min at 37 C to form a gel layer

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High-purity preparations processed in ISO-certified cleanrooms and packaged under nitrogen atmospheres generally demonstrate superior stability profiles compared to products lacking such rigorous quality controls

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