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beta-Klotho deficiency protects against obesity through a crosstalk between liver, microbiota, and brown adipose tissue

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Initially, one injection intramuscularly per week on or around the same day for a maximum of 6 weeks

This stabilizes the transcription factor BACH1, which subsequently upregulates angiogenesis-related factors, including PDGFB, EGFR, FGF2, and FGFR1, ultimately driving vascular regeneration

The intranasal administration in rats typically requires anesthesia, although devices are being developed to circumvent this limitation