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We observed differential morphogenetic responses between the gdh3 / gdh3 mutant and the wild-type strain when grown on proline as the sole carbon and nitrogen source

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After 15 minutes of incubation at room temperature, the transfection mix was added dropwise to the cells, which were cultured in 2 ml of their individual medium, containing 10% FCS and 1% penicillin/streptomycin