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USA 118 , e2019415118 (2021)

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Premalignant melanoma 1/3 of lentigo maligna (Hutchinsons freckle) progress to malignant melanoma after 1015 years

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Proximal jejunum mRNA abundance was determined using a 2-step quantitative real-time PCR (qRT-PCR) protocol on an ABI Prism Sequence Detection System (Applied Biosystems, model 7900 HT) according to the manufacturers instructions