The lower polar phase used for polar metabolite analysis was lyophilized, dissolved in 150 L of 1:1 DDW:MetOH (v/v), centrifuged at 20,000 g for 5 min, transferred to a new tube, and centrifuged again
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Accordingly (and against accepted convention), the contribution of CYP2E1 to ALD pathophysiology may be more complex than originally thought and is likely dependent on the cellular microenvironment or subject to modulation by other signaling pathways 15,37
doi: 10.3389/fphar.2021.681223 107 RobertSMBuckinghamSCCampbellSLRobelSHoltKTOgunrinu-BabarindeTet al
PubMed Central, Li, Li, et al
Common clinical symptoms include irregular uterine bleeding, painful intercourse (dyspareunia), and painful periods (dysmenorrhea) [6,7]