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Flow cytometry Cell cycle analysis of cells cultured in liquid medium was performed as described [28] using a FACS Canto II flow cytometer (BD Bioscienes, San Jose, CA, USA)

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In cultured adipocytes and several mouse models, 5-Amino-1MQ reduced the NNMT activity marker 1-methylnicotinamide and was associated with changes in adiposity, glucose handling, liver histology and aged-muscle performance

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Self-stretching of the extensors and a strengthening program is then gradually introduced

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