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Rev Aquacult 12:17921817 Zhao Y, Xu J, Zhao C, Bao L, Wu K, Feng L, Sun H, Shang S, Hu X, Sun Q, Fu Y (2023) Phytosphingosine alleviates Staphylococcus aureus -induced mastitis by inhibiting inflammatory responses and improving the blood-milk barrier in mice

200 L tissue homogenate was mixed into a tube pre-added with 800 L of ice-cold methanol/water 80% (vol/vol) followed by vortexing rigorously for 1 min, and then centrifuge at ~ 20,160 g for 15 min in a refrigerated centrifuge

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That is, it must have the same active ingredients, strength, and dosage form, be it a pill, liquid, or injection

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