CellRox deep red reagent in a final concentration of 5 M, as well as Hoechst (1:2000) for nuclear staining were added to the wells for 1 hour at 37C
This is because their bodies change with age
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During iron metabolism, less than 10% of the iron demand is met by intestinal absorption, and the remaining iron is exported by ferroportin [4]
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Future efforts focused on mechanistic elucidation, precision microbiota profiling, and longitudinal clinical studies will be crucial for translating these emerging strategies into effective, personalized treatments for hypothyroidism